human fibroblast cell lines  (ATCC)

Journal: Biochemistry and Biophysics Reports

Article Title: Preliminary study of cyto-impedance: Molecular profiling of functional impedance in motility-promoting treatment of normal cells

doi: 10.1016/j.bbrep.2026.102476

Figure Lengend Snippet: EH-P002A promotes wound healing in a dose-dependent manner . A. Molecular structure of EH-P002A. B. No detectable cytotoxicity was observed in HFF-1 or Beas-2B cells upon EH-P002A treatment. C. Wound images from mice treated with EH-P002A at two doses, captured at different time points. D. Wound healing rates on Days 7 and 9. Dose-dependent efficacy on Day 7 was observed.

Article Snippet: Human fibroblast cell line HFF-1 (Cat. No. SCRC-1041, ATCC) and bronchial epithelial cell line Beas-2B (Cat. No. CRL-3588, ATCC) were cultured in Dulbecco's Modified Eagle Medium supplemented with 10 % fetal bovine serum in a humidified 5 % CO2 incubator at 37 °C, and confirmed to be free of mycoplasma contamination.

Techniques:

Journal: Biochemistry and Biophysics Reports

Article Title: Preliminary study of cyto-impedance: Molecular profiling of functional impedance in motility-promoting treatment of normal cells

doi: 10.1016/j.bbrep.2026.102476

Figure Lengend Snippet: Dose-dependent cyto-impedance induced by EH-P002A . A . Wound-healing assay images of HFF-1 cells treated with indicated concentrations of EH-P002A, followed by quantification in the right panel. A promoting effect on wound healing was observed at 8 μM, while overdosage of EH-P002A suppressed the migratory ability. B . Images of migrated HFF-1 cells in transwell assays treated with varying EH-P002A concentrations followed by quantification in the right panel, showing promotion at 8 μM and suppression at higher doses. C. The data of wound-healing assay of Beas-2B cells were quantified, showing a promoting effect at 8–20 μM concentrations, and a suppressive effect at a higher concentration of 80 μM. D . Quantification of the transwell assays of Beas-2B cells confirmed dose-dependent cyto-impedance at 40 and 80 μM concentrations. ∗, P < 0.05; ∗∗, P = 0.001; ∗∗∗, P < 0.001.

Article Snippet: Human fibroblast cell line HFF-1 (Cat. No. SCRC-1041, ATCC) and bronchial epithelial cell line Beas-2B (Cat. No. CRL-3588, ATCC) were cultured in Dulbecco's Modified Eagle Medium supplemented with 10 % fetal bovine serum in a humidified 5 % CO2 incubator at 37 °C, and confirmed to be free of mycoplasma contamination.

Techniques: Wound Healing Assay, Concentration Assay

Journal: Biochemistry and Biophysics Reports

Article Title: Preliminary study of cyto-impedance: Molecular profiling of functional impedance in motility-promoting treatment of normal cells

doi: 10.1016/j.bbrep.2026.102476

Figure Lengend Snippet: Differentially expressed genes in cyto-impedance (6 downregulated, 20 upregulated) . HFF-1 cells were treated with EH-P002 for 48 h. The cellular mRNA levels were assessed via whole-genome expression profiling. A. The downregulated genes during cyto-impedance. B. The upregulated genes associated with cyto-impedance.

Article Snippet: Human fibroblast cell line HFF-1 (Cat. No. SCRC-1041, ATCC) and bronchial epithelial cell line Beas-2B (Cat. No. CRL-3588, ATCC) were cultured in Dulbecco's Modified Eagle Medium supplemented with 10 % fetal bovine serum in a humidified 5 % CO2 incubator at 37 °C, and confirmed to be free of mycoplasma contamination.

Techniques: Expressing

Journal: Macromolecular Bioscience

Article Title: Lignin Nanoparticles Containing Cobalt‐Cyanine Complexes: Potential Multifunctional Platforms for Photoacoustic Imaging and Photothermal Treatment of Bacterial Biofilms in Chronic Wounds

doi: 10.1002/mabi.202500532

Figure Lengend Snippet: Cell compatibility of CoPc‐Lig NPs. A) Cell viability of fibroblasts (black bars) and keratinocytes (white bars) exposed to different concentrations of CoPc‐Lig NPs for 24 h. B) Confocal images showing cell internalization of CoPc‐Lig NPs (0.5 mg/mL) by fibroblasts and keratinocytes. The dark areas in brightfield images represent CoPc‐Lig NPs, while the fluorescence channels show cell nuclei (Blue), and cell cytoskeleton (green).

Article Snippet: Bacterial strains ( Staphylococcus aureus ATCC 6538 and Pseudomonas aeruginosa ATCC 9027) and human fibroblast cell line (ATCC‐SCRC‐1041, HFF‐1) were purchased from the American Type Culture Collection (ATCC LGC Standards, Italy).

Techniques: Fluorescence

Journal: Journal of Microbiology and Biotechnology

Article Title: Mechanism of Anticancer Activity of Cedrol in Epidermal Carcinoma Cells and Its Validation in 3D Artificial Skin Model

doi: 10.4014/jmb.2601.01050

Figure Lengend Snippet: ( A ) 3D Artificial skin models were constructed using HaCaT or A431 cells together with HFF-1 cells and cultured under differentiation conditions for 14 days. ( B ) Histological analysis by H&E staining. Artificial skin models generated with HaCaT or A431 cells were examined for histological properties at each differentiation period (days 0, 7, 14). Scale bars, 60 μm. ( C, D ) Immunofluorescence staining of paraffin sections of artificial skin using keratinocyte differentiation markers, CK10 (red) and loricrin (green). DAPI was used as a nuclear counterstain. Scale bars, 50 μm.

Article Snippet: Human epidermoid carcinoma A431 and human fibroblast HFF-1 cell lines were purchased from American Type Culture Collection (ATCC, USA).

Techniques: Construct, Cell Culture, Staining, Generated, Immunofluorescence